Ashavaid T F, Shalia K K, Nair K G, Dalal J J
Research Laboratories, P.D. Hinduja National Hospital and Medical Research Centre, Mumbai, India.
J Clin Lab Anal. 2000;14(5):230-7. doi: 10.1002/1098-2825(2000)14:5<230::AID-JCLA6>3.0.CO;2-U.
The renin angiotensin system (RAS) controls intrarenal blood pressure and sodium balance, and is an important target for antihypertensive therapy. Several polymorphisms have been identified within genes encoding RAS that may contribute to the development of elevated blood pressure. The relevance of these polymorphisms in hypertension remains controversial. In this study we have examined 105 hypertensive subjects and 192 controls from the Indian population for I/D polymorphism of angiotensin I converting enzyme (ACE) and A(1166)C polymorphism of angiotensin II type I receptor (AT1R) genes by polymerase chain reaction (PCR) and PCR-based restriction enzyme analysis method, respectively. There was no significant difference in the distribution of ACE (I/I, I/D, and D/D) and AT1R (A/A and A/C) genotypes between controls and hypertensive subjects. D allele was significantly associated with an early onset of hypertension and although nonsignificant, the frequency was high in subjects with family history of cardiovascular disorders. C(1166) allele of AT1R did not correlate with the age of onset of hypertension and the frequency was low in subjects with family history. Thus no association was found between ACE and AT1R genotypes and hypertension. However the D allele can be used as a predictor of risk of hypertension in the Indian population.
肾素血管紧张素系统（RAS）控制肾内血压和钠平衡，是抗高血压治疗的重要靶点。在编码RAS的基因中已鉴定出几种多态性，这些多态性可能与血压升高的发生有关。这些多态性在高血压中的相关性仍存在争议。在本研究中，我们分别通过聚合酶链反应（PCR）和基于PCR的限制性酶切分析方法，对105名印度高血压患者和192名对照者进行了血管紧张素I转换酶（ACE）的I/D多态性和血管紧张素II 1型受体（AT1R）基因的A(1166)C多态性检测。对照者与高血压患者之间ACE（I/I、I/D和D/D）和AT1R（A/A和A/C）基因型的分布没有显著差异。D等位基因与高血压的早发显著相关，尽管无统计学意义，但在有心血管疾病家族史的受试者中频率较高。AT1R的C(1166)等位基因与高血压的发病年龄无关，在有家族史的受试者中频率较低。因此，未发现ACE和AT1R基因型与高血压之间存在关联。然而，D等位基因可作为印度人群高血压风险的预测指标。
