Nishio J, Kohsaka H, Shimamura T, Hamuro J, Miyasaka N
Department of Bioregulatory Medicine and Rheumatology, Graduate School, Tokyo Medical and Dental University, Japan.
J Rheumatol. 2001 Feb;28(2):240-4.
Activated macrophages upregulate surface expression of common cytokine receptor gamma chains (gammac, CD132), triggering of which induces secretion of proinflammatory cytokines. Rheumatoid synovial tissues contain a number of macrophages that play a pathogenic role by secreting proinflammatory cytokines. We studied the expression of gammac in the rheumatoid synovial tissues.
Cryosections of synovial tissues from patients with active rheumatoid arthritis (RA) or with osteoarthritis (OA) were stained with an anti-gammac Mab. Single-cell suspensions from the rheumatoid synovial tissues were stained with the same antibody and an anti-CD14 monoclonal antibody (Mab) for 2-color flow cytometric analysis. A soluble form of gammac in synovial fluids collected from rheumatoid or OA joints was quantitated by ELISA.
Rheumatoid synovial tissues, but not the OA tissues, expressed gammac at a high level. Flow cytometric analysis showed that gammac were expressed by virtually all CD 14 positive synovial cells in RA. Synovial fluid derived from the rheumatoid joints contained a high concentration of soluble gammac.
Membrane bound and soluble forms of gammac are abundant in rheumatoid joints. They might play a complex role in the pathology of RA.
活化的巨噬细胞会上调共同细胞因子受体γ链（γc，CD132）的表面表达，触发该受体可诱导促炎细胞因子的分泌。类风湿性滑膜组织含有大量巨噬细胞，这些巨噬细胞通过分泌促炎细胞因子发挥致病作用。我们研究了γc在类风湿性滑膜组织中的表达。
用抗γc单克隆抗体对活动性类风湿关节炎（RA）患者或骨关节炎（OA）患者的滑膜组织冰冻切片进行染色。用相同抗体和抗CD14单克隆抗体对类风湿性滑膜组织的单细胞悬液进行染色，用于双色流式细胞术分析。通过酶联免疫吸附测定法对从类风湿性或OA关节收集的滑液中γc的可溶性形式进行定量。
类风湿性滑膜组织而非OA组织高水平表达γc。流式细胞术分析显示，在RA中几乎所有CD14阳性滑膜细胞均表达γc。类风湿性关节的滑液含有高浓度的可溶性γc。
γc的膜结合形式和可溶性形式在类风湿性关节中大量存在。它们可能在RA的病理过程中发挥复杂作用。
