Shono M, Wada M, Hara Y, Fujii T
Japanese International Research Center for Agricultural Sciences-Okinawa Subtropical Station, Ishigaki, Japan.
Biochim Biophys Acta. 2001 Mar 9;1511(1):193-9. doi: 10.1016/s0005-2736(01)00266-8.
We cloned novel Na(+)-ATPase (HANA) cDNA from marine alga Heterosigma akashiwo. The full-length HANA cDNA was 4467 bp long and coded for a 1330 amino acid protein with a molecular weight of 146,306. The deduced product exhibited around 40% identity in amino acids with Na(+)/K(+)-ATPase alpha-subunits. A hydrophilic sequence of 285 amino acid residues that showed no homology with any sequence listed in databases existed in the M7--M8 junction of HANA. This is the first report on the primary structure of putative Na(+)-transporting ATPase from plant cells.
我们从海洋藻类赤潮异弯藻中克隆出了新型钠-ATP酶（HANA）的cDNA。HANA cDNA全长4467 bp，编码一个由1330个氨基酸组成的蛋白质，分子量为146,306。推导的产物与钠/钾-ATP酶α亚基在氨基酸水平上具有约40%的同一性。在HANA的M7-M8连接处存在一段285个氨基酸残基的亲水区序列，该序列与数据库中列出的任何序列均无同源性。这是关于植物细胞中假定的钠转运ATP酶一级结构的首次报道。
