Song P, Zhao Z Q
Shanghai Institute of Physiology, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
Neurosci Res. 2001 Mar;39(3):281-6. doi: 10.1016/s0168-0102(00)00226-1.
Glial response to chronic morphine treatment was examined by immunohistochemistry of glial fibrillary acidic protein (GFAP), a specific marker for astroglial cells. Systemic administration of morphine (50 mg/kg, i.p.) once daily for 9 consecutive days led to significant increase in GFAP immunostaining density in the spinal cord, posterior cingulate cortex and hippocampus but not in the thalamus. This increase was attributed primarily to hypertrophy of astroglial cells rather than their proliferation or migration. When chronic morphine (20 microg/2 microl, i.t.) was delivered in combination with fluorocitrate (1 nmol/1 microl, i.t.), a specific and reversible inhibitor of glial cells, spinal tolerance to morphine analgesia was partly but significantly attenuated as measured by behavioural test and the increase in spinal GFAP immunostaining was also greatly blocked. The present investigation provides the first evidence for the role of glial cells in the development of morphine tolerance in vivo.
通过对胶质纤维酸性蛋白（GFAP，星形胶质细胞的一种特异性标志物）进行免疫组织化学检测，研究了胶质细胞对慢性吗啡治疗的反应。连续9天每天一次腹腔注射吗啡（50毫克/千克），导致脊髓、后扣带回皮质和海马体中GFAP免疫染色密度显著增加，但丘脑未出现这种情况。这种增加主要归因于星形胶质细胞的肥大，而非其增殖或迁移。当将慢性吗啡（20微克/2微升，鞘内注射）与氟柠檬酸（1纳摩尔/1微升，鞘内注射，一种胶质细胞特异性且可逆的抑制剂）联合使用时，通过行为测试测量发现，吗啡镇痛的脊髓耐受性部分但显著减弱，同时脊髓GFAP免疫染色的增加也被大大阻断。本研究首次为胶质细胞在体内吗啡耐受性形成中的作用提供了证据。
