Contzen M, Moore E R, Blümel S, Stolz A, Kämpfer P
Institut für Mikrobiologie, Universität Stuttgart, Germany.
Syst Appl Microbiol. 2000 Dec;23(4):487-93. doi: 10.1016/S0723-2020(00)80022-3.
The taxonomic status of a gram-negative, oxidase positive rod (strain S1) able to degrade 4-aminobenzenesulfonate was studied using a polyphasic approach. Chemotaxonomic investigations of quinones and polar lipids established the allocation of this strain to the beta-subclass of the Proteobacteria and revealed similarities to Hydrogenophaga palleronii. 16S rRNA sequence comparisons demonstrated that this strain clusters phylogenetically with H. palleronii and H. taeniospiralis, but clearly represents a new species. The fatty acid patterns and substrate utilization profile displayed similarity to the characteristics of the four validly published species of Hydrogenophaga, although clear differentiating characters were also observed. No close similarities between the type strains of H. palleronii and H. taeniospiralis were detected in hybridization experiments with the genomic DNAs. On basis of these results, the new species Hydrogenophaga intermedia sp. nov. is proposed, with the type strain S1T (= DSM 5680).
采用多相分类方法对一株能够降解4-氨基苯磺酸盐的革兰氏阴性、氧化酶阳性杆菌（菌株S1）的分类地位进行了研究。对醌类和极性脂质的化学分类学研究确定了该菌株属于变形菌纲β亚类，并显示出与苍白嗜氢菌的相似性。16S rRNA序列比较表明，该菌株在系统发育上与苍白嗜氢菌和螺旋嗜氢菌聚类，但明显代表一个新物种。脂肪酸模式和底物利用谱与已有效发表的四种嗜氢菌的特征相似，尽管也观察到明显的鉴别特征。在与基因组DNA的杂交实验中，未检测到苍白嗜氢菌和螺旋嗜氢菌模式菌株之间的密切相似性。基于这些结果，提出了中间嗜氢菌新种，模式菌株为S1T（=DSM 5680）。
