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与大西洋鲑(Salmo salar L.)鳃相关的肉食杆菌的鉴定与特性分析。

Identification and characterization of carnobacteria associated with the gills of Atlantic salmon (Salmo salar L.).

作者信息

Ringø E, Holzapfel W

机构信息

Department of Arctic Veterinary Medicine, the Norwegian School of Veterinary Science, Tromsø.

出版信息

Syst Appl Microbiol. 2000 Dec;23(4):523-7. doi: 10.1016/s0723-2020(00)80026-0.

DOI:10.1016/s0723-2020(00)80026-0
PMID:11249022
Abstract

Using the surface plate technique, the population level of aerobic bacteria, occurring in the gills of Atlantic salmon (Salmo salar L.), was determined to be approximately 3 x 10(4) g(-1). Of 100 isolates investigated, 58 were gram-negative. Out of the 42 gram-positive isolates, 26 belonged to the carnobacteria, of which ten were further identified on the basis of 16S rDNA sequence analysis and AFLP fingerprinting. All were identified as Carnobacterium piscicola-like. These carnobacteria strains were also screened for their ability to produce growth inhibitory compounds active against the fish pathogens Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Vibrio salmonicida. Nine out of the ten C. piscicola isolates tested strongly inhibited growth of the three pathogens.

摘要

采用平板技术测定了大西洋鲑(Salmo salar L.)鳃中需氧细菌的数量水平,约为3×10⁴ g⁻¹。在所研究的100株分离菌中,58株为革兰氏阴性菌。在42株革兰氏阳性分离菌中,26株属于肉杆菌属,其中10株通过16S rDNA序列分析和AFLP指纹图谱进一步鉴定。所有菌株均被鉴定为类嗜鱼肉杆菌。还对这些肉杆菌菌株产生对鱼类病原菌杀鲑气单胞菌亚种鲑气单胞菌、鳗弧菌和杀鲑弧菌具有生长抑制活性化合物的能力进行了筛选。在所测试的10株嗜鱼肉杆菌分离菌中,有9株强烈抑制了这三种病原菌的生长。

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