Ringø E, Bendiksen H R, Wesmajervi M S, Olsen R E, Jansen P A, Mikkelsen H
Department of Arctic Veterinary Medicine, The Norwegian School of Veterinary Science, Tromsø, Norway.
J Appl Microbiol. 2000 Aug;89(2):317-22. doi: 10.1046/j.1365-2672.2000.01116.x.
The present study reports the effect of excessive handling stress and starvation on the lactic acid bacteria associated with the digestive tract of Atlantic salmon (Salmo salar L.). A relatively low population level (approximately 2 x 103 bacteria per gram wet tissue) of viable adherent heterotrophic bacteria was associated with the digestive tract (foregut, midgut and hindgut). Of the 752 bacterial isolates isolated from diet, water and the digestive tract, 201 isolates belonged to the carnobacteria. Of these isolates, one from the diet, one from the rearing water and 80 from the gastrointestinal tract, were further identified on the basis of 16S rDNA sequence analysis. All these isolates were identified as being Carnobacterium piscicola-like. Daily repeated stress and starvation of the fish over 11 d had no influence on the total culturable bacterial numbers or population level of C. piscicola associated with the digestive tract. C. piscicola-like isolates colonizing the various intestinal regions (foregut, midgut and hindgut) were also screened for their ability to produce growth inhibitory compounds active against the fish pathogen Aeromonas salmonicida. Of the 199 C. piscicola isolates tested, 139 inhibited growth of the pathogen.
本研究报告了过度处理应激和饥饿对大西洋鲑（Salmo salar L.）消化道相关乳酸菌的影响。消化道（前肠、中肠和后肠）中存活的粘附性异养细菌数量相对较低（每克湿组织约2×10³个细菌）。从饲料、水和消化道分离出的752株细菌分离物中，有201株属于肉杆菌属。在这些分离物中，根据16S rDNA序列分析，进一步鉴定了1株来自饲料、1株来自养殖水和80株来自胃肠道的分离物。所有这些分离物均被鉴定为类嗜鱼肉杆菌。在11天内对鱼进行每日重复应激和饥饿处理，对与消化道相关的可培养细菌总数或嗜鱼肉杆菌的种群水平没有影响。还筛选了定殖在不同肠道区域（前肠、中肠和后肠）的类嗜鱼肉杆菌分离物产生对鱼类病原菌杀鲑气单胞菌有活性的生长抑制化合物的能力。在测试的199株嗜鱼肉杆菌分离物中，有139株抑制了病原菌的生长。
