• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

RNA-polymerase III-driven expression cassettes in human gene therapy.

作者信息

Medina M F, Joshi S

机构信息

Department of Medical Genetics and Microbiology, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 3E2, Canada.

出版信息

Curr Opin Mol Ther. 1999 Oct;1(5):580-94.

PMID:11249665
Abstract

tRNAs, U6 small nuclear RNA and adenovirus virus associated RNAs are transcribed by RNA polymerase III. These RNAs are expressed in high levels and possess increased stability due to their small, compact size. Promoters expressing these RNAs have therefore been used to express a variety of interfering RNAs, including ribozymes, antisense RNAs and sense or decoy RNAs. Intracellular expression and activity of RNA polymerase III-driven interfering RNAs have been demonstrated in several studies. This review will summarize the design and application of RNA polymerase III-driven expression cassettes in human gene therapy using retroviral vectors as the primary method of gene delivery.

摘要

相似文献

1
RNA-polymerase III-driven expression cassettes in human gene therapy.
Curr Opin Mol Ther. 1999 Oct;1(5):580-94.
2
Retroviral vectors designed for targeted expression of RNA polymerase III-driven transcripts: a comparative study.用于RNA聚合酶III驱动转录本靶向表达的逆转录病毒载体：一项比较研究。
Gene. 1996 Jun 1;171(2):203-8. doi: 10.1016/0378-1119(96)00075-3.
3
Improved accumulation and activity of ribozymes expressed from a tRNA-based RNA polymerase III promoter.基于tRNA的RNA聚合酶III启动子表达的核酶的积累和活性得到改善。
Nucleic Acids Res. 1995 Jun 25;23(12):2259-68. doi: 10.1093/nar/23.12.2259.
4
Utility of Epstein-Barr virus-encoded small RNA promoters for driving the expression of fusion transcripts harboring short hairpin RNAs.爱泼斯坦-巴尔病毒编码的小RNA启动子用于驱动携带短发夹RNA的融合转录本表达的效用。
Gene Ther. 2008 Feb;15(3):191-202. doi: 10.1038/sj.gt.3303055. Epub 2007 Nov 1.
5
Inhibition of HIV-1 infection by lentiviral vectors expressing Pol III-promoted anti-HIV RNAs.表达由RNA聚合酶III介导的抗HIV RNA的慢病毒载体对HIV-1感染的抑制作用
Mol Ther. 2003 Aug;8(2):196-206. doi: 10.1016/s1525-0016(03)00165-5.
6
Localized expression of small RNA inhibitors in human cells.小RNA抑制剂在人细胞中的局部表达。
Mol Ther. 2003 Feb;7(2):237-47. doi: 10.1016/s1525-0016(02)00038-2.
7
Inhibition of HIV-1 replication with designed miRNAs expressed from RNA polymerase II promoters.用从RNA聚合酶II启动子表达的设计性微小RNA抑制HIV-1复制。
Gene Ther. 2007 Nov;14(21):1503-12. doi: 10.1038/sj.gt.3303011. Epub 2007 Sep 6.
8
The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization.表达盒通过控制核酶在细胞内的定位来决定其在哺乳动物细胞中的功能活性。
RNA. 1997 Jan;3(1):75-88.
9
Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes.利用逆转录病毒微小RNA基因的紧凑结构表达短发夹RNA。
Nucleic Acids Res. 2017 Sep 29;45(17):e154. doi: 10.1093/nar/gkx653.
10
Interference between effector RNAs expressed from conventional dual-function anti-HIV retroviral vectors can be circumvented using dual-effector-cassette retroviral vectors.
Hum Gene Ther. 1999 Feb 10;10(3):449-62. doi: 10.1089/10430349950018896.

引用本文的文献

1
SINEs as Potential Expression Cassettes: Impact of Deletions and Insertions on Polyadenylation and Lifetime of B2 and Ves SINE Transcripts Generated by RNA Polymerase III.SINEs 作为潜在的表达盒：RNA 聚合酶 III 生成的 B2 和 Ves SINE 转录本的多聚腺苷酸化和寿命受缺失和插入的影响。
Int J Mol Sci. 2023 Sep 27;24(19):14600. doi: 10.3390/ijms241914600.
2
Empower multiplex cell and tissue-specific CRISPR-mediated gene manipulation with self-cleaving ribozymes and tRNA.利用自我切割核酶和tRNA实现多重细胞和组织特异性CRISPR介导的基因操作。
Nucleic Acids Res. 2017 Mar 17;45(5):e28. doi: 10.1093/nar/gkw1048.
3
Viral noncoding RNAs: more surprises.
病毒非编码RNA：更多惊喜
Genes Dev. 2015 Mar 15;29(6):567-84. doi: 10.1101/gad.259077.115.
4
Versatile microRNA biogenesis in animals and their viruses.动物及其病毒中多功能的微小RNA生物合成
RNA Biol. 2014;11(6):673-81. doi: 10.4161/rna.28985. Epub 2014 May 7.
5
An RNAi-based approach to down-regulate a gene family in vivo.一种基于 RNAi 的体内下调基因家族的方法。
PLoS One. 2013 Nov 12;8(11):e80312. doi: 10.1371/journal.pone.0080312. eCollection 2013.
6
RNA-programmed genome editing in human cells.人类细胞中的RNA编程基因组编辑。
Elife. 2013 Jan 29;2:e00471. doi: 10.7554/eLife.00471.
7
Alternative miRNA biogenesis pathways and the interpretation of core miRNA pathway mutants.替代 miRNA 生物发生途径和核心 miRNA 途径突变体的解释。
Mol Cell. 2011 Sep 16;43(6):892-903. doi: 10.1016/j.molcel.2011.07.024.
8
HIV-1 RT-dependent DNAzyme expression inhibits HIV-1 replication without the emergence of escape viruses.HIV-1 RT 依赖性 DNA 酶表达抑制 HIV-1 复制，而不会出现逃逸病毒。
Nucleic Acids Res. 2011 Jan;39(2):589-98. doi: 10.1093/nar/gkq794. Epub 2010 Sep 9.
9
RNA Interference inhibits hepatitis B virus of different genotypes in vitro and in vivo.RNA 干扰抑制不同基因型乙型肝炎病毒在体外和体内的复制。
BMC Microbiol. 2010 Aug 10;10:214. doi: 10.1186/1471-2180-10-214.
10
Comparative studies of various artificial microRNA expression vectors for RNAi in mammalian cells.各种人工 microRNA 表达载体在哺乳动物细胞中 RNAi 的比较研究。
Mol Biotechnol. 2010 Sep;46(1):34-40. doi: 10.1007/s12033-010-9264-7.