Lachmann R H, Efstathiou S
Department of Medicine, Box 157, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 2QQ, UK.
Curr Opin Mol Ther. 1999 Oct;1(5):622-32.
In developing any viral gene delivery vector there are two fundamental problems which need to be addressed. Firstly, replication disabled vectors which will be safe for clinical use must be constructed, and secondly, strategies for obtaining appropriate transgene expression in vector transduced cells must be devised. In this review, the progress which has been made in developing herpes simplex virus (HSV)-based gene delivery vectors is discussed, as are the experimental results which have been obtained using these vectors for gene delivery in tissue culture cells and animal models.
在开发任何病毒基因递送载体时，有两个基本问题需要解决。首先，必须构建对临床使用安全的复制缺陷型载体，其次，必须设计在载体转导细胞中获得适当转基因表达的策略。在这篇综述中，将讨论在开发基于单纯疱疹病毒（HSV）的基因递送载体方面取得的进展，以及使用这些载体在组织培养细胞和动物模型中进行基因递送所获得的实验结果。
