Dasgupta A, Galvao Neto A L, Wells A, Olsen M, Actor J K
Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, Houston, TX 77030, USA.
Clin Chim Acta. 2001 Mar;305(1-2):175-9. doi: 10.1016/s0009-8981(01)00377-1.
Traditional Chinese medicines are readily available without prescription from herbal drug stores. One such Chinese medicine, Chan Su, which is prepared from the skin gland of toads, has cardiotonic effect due to bufadienolides. Here we report rapid detection of the presence of Chan Su in blood using the fluorescence polarization immunoassay for digitoxin. In our study mice were fed with a dose of 75 mg/kg of Chan Su and blood was drawn before, and 1 and 2 h after feeding. We observed significant digitoxin-like immunoreactivity in the sera. For example in one mouse the digitoxin-like immunoreactivity was undetectable before feeding with Chan Su, but was 19.7 ng/ml 1 h and 8.8 ng/ml 2 h afterwards. The apparent half-life of Chan Su is approximately 1 h in mice. In another experiment, we studied protein binding of Chan Su by measuring total and free Chan Su concentrations (ultrafiltrate prepared by using Centrifree Micropartition Filter, molecular weight cutoff: 30000 Da). Chan Su was strongly bound to serum proteins. We observed higher free fraction in uremic sera and sera from patients with liver disease. We identified albumin as one of the proteins that bind Chan Su in serum.
中药在草药药店无需处方即可轻易买到。一种这样的中药蟾酥，是由蟾蜍的皮肤腺体制成的，由于蟾毒配基而具有强心作用。在此我们报告使用地高辛荧光偏振免疫分析法快速检测血液中蟾酥的存在情况。在我们的研究中，给小鼠喂食75毫克/千克剂量的蟾酥，并在喂食前、喂食后1小时和2小时采集血液。我们在血清中观察到显著的地高辛样免疫反应性。例如，在一只小鼠中，喂食蟾酥前未检测到地高辛样免疫反应性，但在喂食后1小时为19.7纳克/毫升，2小时后为8.8纳克/毫升。蟾酥在小鼠体内的表观半衰期约为1小时。在另一项实验中，我们通过测量总蟾酥浓度和游离蟾酥浓度（使用截留分子量为30000道尔顿的Centrifree微滤膜过滤器制备的超滤液）来研究蟾酥与蛋白质的结合情况。蟾酥与血清蛋白紧密结合。我们在尿毒症血清和肝病患者的血清中观察到较高的游离分数。我们确定白蛋白是血清中与蟾酥结合的蛋白质之一。
