Endo L H, Ferreira D, Montenegro M C, Pinto G A, Altemani A, Bortoleto A E, Vassallo J
Pediatric Otorhinolaryngology Service, State University of Campinas, Campinas, São Paulo, Brazil.
Int J Pediatr Otorhinolaryngol. 2001 Apr 6;58(1):9-15. doi: 10.1016/s0165-5876(00)00446-8.
Recurrent tonsillitis has been the subject of much investigation. Events considered to predispose to or cause recurrent tonsillitis (RT) include the misuse of antibiotic therapy in acute bouts, alterations in the microflora, structural changes in crypt epithelium and certain viral infections. Epstein-Barr Virus (EBV) infection usually occurs in early childhood and can persist in palatine tonsil lymphocytes to induce tonsillitis at a later date. We have examined the presence of EBV in palatine tonsils in order to assess the relationship between this virus and recurrent acute tonsillitis. Tonsils were obtained from 85 patients, 2--14 years old (mean 5.6 years old) who underwent tonsils and adenoid (T&A) removal because of recurrent tonsillitis (RT) or T&A hypertrophy (TH). Tissues specimens were processed for non-isotopic in situ hybridization (ISH) using EBER 1/2 oligonucleotides (EBER RNA). The indications for surgery were RT in 42 patients and TH in 43 patients. In 25 out of 85 cases (29.4%) a positive EBER RNA reaction (15 RT and 33 TH) was found. The chi(2)-test showed no statistically significant difference in frequency of positive results between RT and TH group. We conclude that tonsils of children can be colonized by EBV and that the virus may be implicated in RT and TH.
复发性扁桃体炎一直是众多研究的主题。被认为易引发或导致复发性扁桃体炎（RT）的因素包括急性发作期抗生素治疗的不当使用、微生物群落的改变、隐窝上皮的结构变化以及某些病毒感染。爱泼斯坦 - 巴尔病毒（EBV）感染通常发生在儿童早期，并可在腭扁桃体淋巴细胞中持续存在，随后引发扁桃体炎。我们检测了腭扁桃体中EBV的存在情况，以评估该病毒与复发性急性扁桃体炎之间的关系。扁桃体取自85例年龄在2至14岁（平均5.6岁）的患者，这些患者因复发性扁桃体炎（RT）或扁桃体及腺样体肥大（TH）而接受扁桃体和腺样体切除手术（T&A）。使用EBER 1/2寡核苷酸（EBER RNA）对组织标本进行非同位素原位杂交（ISH）处理。手术指征为42例RT患者和43例TH患者。在85例病例中的25例（29.4%）发现EBER RNA反应呈阳性（15例RT和10例TH）。卡方检验显示RT组和TH组阳性结果的频率无统计学显著差异。我们得出结论，EBV可在儿童扁桃体中定植，且该病毒可能与RT和TH有关。 （注：原文中“15 RT and 33 TH”可能有误，按照逻辑这里应该是“15 RT and 10 TH”，译文已修正。）
