Dong H, Lin W, Zhang C K, Xiong H, Fu G, Jin W R, Chen R, Chen Z, Qi Z T, Huang G M
Chinese National Human Genome Center at Shanghai, 351 Guo Shou Jing Road, Zhangjiang High Tech Park, Shanghai 201203, P.R. China.
Gene. 2001 Feb 21;264(2):187-96. doi: 10.1016/s0378-1119(01)00335-3.
Chromatin assembly factor-1 (CAF-1) plays essential roles in eukaryotic chromatin assembly during DNA replication (Smith and Stillman, 1989. Cell 58, 15-25), (Krude, 1999. Eur. J. Biochem. 263, 1-5). Its p150 subunit, involved in interaction with histone H3 and H4, is critical to the CAF-1 nucleosome assembly activity. In this study, we sequenced a 96-kb genomic DNA region that includes a 42.8-kb CAF-1 p150 subunit gene (CHAF1A), and a 41.1-kb EEN gene. A scripted bioinformatics analysis pipeline (research agent) has been set up to annotate the BAC sequence with a set of integrated algorithms. The CAF-1 p150 subunit gene contains 15 exons and 14 introns. The promoter region is characterized by deletional analyses, revealing a potential repressor. Tissue-correlated alternative splicing forms of the transcript was initially identified by EST clustering analysis, then confirmed by RT-PCR which resulted more splicing forms than computational prediction.
染色质组装因子-1（CAF-1）在DNA复制过程中的真核染色质组装中发挥着重要作用（Smith和Stillman，1989年。《细胞》58卷，第15 - 25页），（Krude，1999年。《欧洲生物化学杂志》263卷，第1 - 5页）。其p150亚基参与与组蛋白H3和H4的相互作用，对CAF-1核小体组装活性至关重要。在本研究中，我们对一个96 kb的基因组DNA区域进行了测序，该区域包括一个42.8 kb的CAF-1 p150亚基基因（CHAF1A）和一个41.1 kb的EEN基因。已建立一个脚本化的生物信息学分析流程（研究代理），用一组集成算法对BAC序列进行注释。CAF-1 p150亚基基因包含15个外显子和14个内含子。通过缺失分析对启动子区域进行了表征，揭示了一个潜在的阻遏物。转录本的组织相关可变剪接形式最初通过EST聚类分析确定，然后通过RT-PCR得到证实，RT-PCR产生的剪接形式比计算预测的更多。
