Institute of Biostructures and Bioimaging, Consiglio Nazionale delle Ricerche (IBB-CNR), Via Mezzocannone, 16, 80134 Naples, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, 81100 Caserta, Italy.
Int J Mol Sci. 2022 Feb 15;23(4):2160. doi: 10.3390/ijms23042160.
The Chromatin Assembly Factor 1 is a heterotrimeric complex responsible for the nucleosome assembly during DNA replication and DNA repair. In humans, the largest subunit P150 is the major actor of this process. It has been recently considered as a tumor-associated protein due to its overexpression in many malignancies. Structural and functional studies targeting P150 are still limited and only scarce information about this subunit is currently available. Literature data and bioinformatics analysis assisted the identification of a stable DNA binding domain, encompassing residues from 721 to 860 of P150 within the full-length protein. This domain was recombinantly produced and in vitro investigated. An acidic region modulating its DNA binding ability was also identified and characterized. Results showed similarities and differences between the P150 and its yeast homologue, namely Cac-1, suggesting that, although sharing a common biological function, the two proteins may also possess different features.
染色质组装因子 1 是一种异三聚体复合物，负责在 DNA 复制和 DNA 修复过程中组装核小体。在人类中，最大的亚基 P150 是该过程的主要参与者。由于其在许多恶性肿瘤中的过度表达，最近它被认为是一种与肿瘤相关的蛋白质。针对 P150 的结构和功能研究仍然有限，目前关于该亚基的信息还很稀缺。文献数据和生物信息学分析协助鉴定了一个稳定的 DNA 结合域，该域包含全长蛋白中 P150 的 721 到 860 位残基。该结构域被重组产生并在体外进行了研究。还鉴定并表征了一个调节其 DNA 结合能力的酸性区域。结果表明，P150 与其酵母同源物 Cac-1 之间存在相似性和差异，这表明尽管它们具有共同的生物学功能，但这两种蛋白质可能也具有不同的特征。
