Feucht A, Lewis P J
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Gene. 2001 Feb 21;264(2):289-97. doi: 10.1016/s0378-1119(01)00338-9.
The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytological marker to monitor protein localisation in live cells. Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions. In this work we report the design and use of vectors containing some of these spectral variants of GFP for use in the Gram positive bacterium Bacillus subtilis. These vectors complement those previously described (Lewis and Marston, 1999. Gene 227, 101-109) to provide a large suite of plasmid vectors for use in this and other related Gram positive organisms. Using these vectors we have been able to directly demonstrate the sequential assembly/disassembly of proteins involved in the generation of cellular asymmetry during development.
内在荧光的绿色荧光蛋白已在许多实验室用作细胞学标记，以监测活细胞中的蛋白质定位。随后报道了多种经过光谱修饰的突变体版本以及来自其他物种的新型荧光蛋白，并用于标记具有多种荧光蛋白融合体的细胞。在这项工作中，我们报告了含有一些这些GFP光谱变体的载体的设计和使用，用于革兰氏阳性细菌枯草芽孢杆菌。这些载体补充了先前描述的载体（Lewis和Marston，1999年。基因227，101-109），以提供一大套用于这种和其他相关革兰氏阳性生物的质粒载体。使用这些载体，我们能够直接证明在发育过程中参与细胞不对称性产生的蛋白质的顺序组装/拆卸。
