Department of Microbial Genetics, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia.
Int J Mol Sci. 2020 Jun 25;21(12):4513. doi: 10.3390/ijms21124513.
Peptidoglycan is generally considered one of the main determinants of cell shape in bacteria. In rod-shaped bacteria, cell elongation requires peptidoglycan synthesis to lengthen the cell wall. In addition, peptidoglycan is synthesized at the division septum during cell division. Sporulation of begins with an asymmetric cell division. Formation of the sporulation septum requires almost the same set of proteins as the vegetative septum; however, these two septa are significantly different. In addition to their differences in localization, the sporulation septum is thinner and it contains SpoIIE, a crucial sporulation specific protein. Here we show that peptidoglycan biosynthesis is linked to the cell division machinery during sporulation septum formation. We detected a direct interaction between SpoIIE and GpsB and found that both proteins co-localize during the early stages of asymmetric septum formation. We propose that SpoIIE is part of a multi-protein complex which includes GpsB, other division proteins and peptidoglycan synthesis proteins, and could provide a link between the peptidoglycan synthesis machinery and the complex morphological changes required for forespore formation during sporulation.
肽聚糖通常被认为是细菌细胞形状的主要决定因素之一。在杆状细菌中，细胞伸长需要肽聚糖合成来延长细胞壁。此外，肽聚糖在细胞分裂时在分裂隔膜处合成。 的孢子形成始于不对称细胞分裂。形成孢子隔膜需要与营养期隔膜几乎相同的一组蛋白质；然而，这两个隔膜有很大的不同。除了它们在定位上的差异外，孢子隔膜更薄，并且它包含 SpoIIE，这是一种关键的孢子特异性蛋白质。在这里，我们表明肽聚糖生物合成与孢子隔膜形成过程中的细胞分裂机制有关。我们检测到 SpoIIE 与 GpsB 之间的直接相互作用，并发现这两种蛋白质在不对称隔膜形成的早期阶段共定位。我们提出 SpoIIE 是一个多蛋白复合物的一部分，该复合物包括 GpsB、其他分裂蛋白和肽聚糖合成蛋白，并可能为肽聚糖合成机制与孢子形成过程中前孢子形成所需的复杂形态变化之间提供联系。
