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[刚地弓形虫的抗原结构。III. 与纯化抗原相比人类血清的反应]

[The antigenic structure of Toxoplasma gondii. III. Behavior of human sera as compared with purified antigens].

作者信息

Spanó C, LA Rosa G, LA Licata R

出版信息

Boll Ist Sieroter Milan. 1975;54(1):36-44.

PMID:1125110
Abstract

263 human serum samples obtained from 9 controls and 42 patients with clinical diagnosis suggestive for toxoplasmosis were examined by means of Sabin and Feldman's dye test (DT) in comparison with complement fixation test (CF) with a whole toxoplasma antigen. 6 out of 9 controls and 21 out of 42 patients showed analogous behaviour in both methods while the remaining ones behaved differently. 24 out of the 42 patients as well as the 9 controls were chosen between these two groups (showing concordant or discordant DT/CF behaviour) to determine the presence or absence of anti-"polysaccharide" and anti-"protein" antibodies against purified antigens by means of CF test. Presence of anti-polysaccharide antibodies was shown in 15 subjects (4 out of 17 "DT/CF discordant" groups) and antiprotien antibodies in 8 patients (2 and 6 respectively in the two groups). Antibodies against these two purified antigens were never shown in the 9 normal people. Critical as well as hypothetical implications concerning the results so far obtained are discussed.

摘要

从9名对照者和42名临床诊断提示弓形虫病的患者中获取了263份人血清样本,采用萨宾和费尔德曼染色试验(DT)进行检测,并与使用全弓形虫抗原的补体结合试验(CF)进行比较。9名对照者中有6名,42名患者中有21名在两种方法中表现类似,其余的表现不同。从这两组(DT/CF行为一致或不一致)中选取了42名患者中的24名以及9名对照者,通过CF试验来确定针对纯化抗原的抗“多糖”和抗“蛋白质”抗体的存在与否。15名受试者(17个“DT/CF不一致”组中的4个)显示存在抗多糖抗体,8名患者(两组分别为2名和6名)显示存在抗蛋白质抗体。9名正常人中从未显示出针对这两种纯化抗原的抗体。讨论了迄今所获结果的关键及假设意义。

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