Ferrucci M
Quad Sclavo Diagn. 1980 Jun;16(2):176-92.
Toxoplasma infection stimulates a very rich answer in circulating antibodies, which are easily detected by many serological tests. Many years elapsed since 1948, when Sabin and Feldmann [28] carried out for the first time a dye test which is, still at present, the chief reference test for the numerous techniques which have been subsequently available. The aim of the present review, which is largely based on personal experiences, is to draw some conclusions on the meaning and usefulness of these tests, and on the best way to express their results. Though a special attention has been allowed to techniques using a whole cellular antigen (dye test, immunofluorescence, direct agglutination test) some useful hints are also given on CF, IHA and ELISA, which employ an extractive antigen. A special stress is layed on the usefulness, or, rather, the need to express results, whenever possible, in IU (International Units), in order to attribute a standard comparable meaning to the tests.
弓形虫感染会刺激循环抗体产生非常丰富的反应,许多血清学检测方法都能轻易检测到这些抗体。自1948年萨宾和费尔德曼[28]首次进行染料试验以来,已经过去了许多年,该试验至今仍是众多后续可用技术的主要参考试验。本综述主要基于个人经验,旨在就这些检测方法的意义和实用性以及表达其结果的最佳方式得出一些结论。尽管对使用全细胞抗原的技术(染料试验、免疫荧光、直接凝集试验)给予了特别关注,但也给出了一些关于补体结合试验(CF)、间接血凝试验(IHA)和酶联免疫吸附测定(ELISA)(这些试验使用提取抗原)的有用提示。特别强调了尽可能以国际单位(IU)来表达结果的实用性,或者更确切地说是必要性,以便赋予这些检测方法一个可比较的标准意义。