染色质的重建：染色体蛋白质的重新结合模式。

Reconstitution of chromatin: mode of reassociation of chromosomal proteins.

作者信息

Chae C B

出版信息

Biochemistry. 1975 Mar 11;14(5):900-6. doi: 10.1021/bi00676a005.

Abstract

The mode of reassociation of Ehrlich ascites histones and non-histone proteins during chromatin reconstitution was studied by sodium dodecyl sulfate polyacryl-amide gel electrophoresis. In the procedure of Bekhor et al. (I. Bekhor, G. M. Kung, and J. Bonner, (1969), J. Mol. Biol. 39, 351) most of histones and non-histone proteins reassociate with DNA in the last dialysis step of the dissociated chromatin, that is the dialysis of the chromatin in 0.4 M NaCl-5 M urea against a dilute buffer. The reassociation of histones and non-histone proteins with DNA is more gradual in the procedure of L. Kleiman and R.-C. C. Huang [(1972), J. Mol. Biol. 64, 1]. However, in both procedures the bulk of the Ehrlich ascites non-histone proteins reassociate with DNA after the binding of histones to DNA. There are small amounts of non-histone proteins which reassociate with DNA before and at the same time as histones reassociate with DNA.

摘要

通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳研究了艾氏腹水组蛋白和非组蛋白在染色质重构过程中的重新结合模式。在贝霍尔等人（I. 贝霍尔、G. M. 孔和J. 邦纳，（1969年），《分子生物学杂志》第39卷，第351页）的实验步骤中，大部分组蛋白和非组蛋白在解离染色质的最后透析步骤中与DNA重新结合，即把染色质置于0.4 M氯化钠 - 5 M尿素中，然后对稀缓冲液进行透析。在L. 克莱曼和R.-C. C. 黄（（1972年），《分子生物学杂志》第64卷，第1页）的实验步骤中，组蛋白和非组蛋白与DNA的重新结合更为渐进。然而，在这两种实验步骤中，艾氏腹水非组蛋白的大部分都是在组蛋白与DNA结合之后与DNA重新结合的。有少量非组蛋白在组蛋白与DNA重新结合之前以及与组蛋白同时与DNA重新结合。