双链RNA介导的转录后基因沉默

Post-transcriptional gene silencing by double-stranded RNA.

作者信息

Hammond S M, Caudy A A, Hannon G J

机构信息

Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.

出版信息

Nat Rev Genet. 2001 Feb;2(2):110-9. doi: 10.1038/35052556.

DOI:10.1038/35052556

PMID:11253050

Abstract

Imagine being able to knock out your favourite gene with only a day's work. Not just in one model system, but in virtually any organism: plants, flies, mice or cultured cells. This sort of experimental dream might one day become reality as we learn to harness the power of RNA interference, the process by which double-stranded RNA induces the silencing of homologous endogenous genes. How this phenomenon works is slowly becoming clear, and might help us to develop an effortless tool to probe gene function in cells and animals.

摘要

想象一下，只需一天的工作就能敲除你最喜欢的基因。不仅在一种模型系统中，而且几乎在任何生物体中：植物、果蝇、小鼠或培养细胞。随着我们学会利用RNA干扰的力量，这种实验梦想有一天可能会成为现实，RNA干扰是一种双链RNA诱导同源内源基因沉默的过程。这种现象是如何起作用的正逐渐变得清晰，并且可能有助于我们开发一种轻松的工具来探究细胞和动物中的基因功能。