存在于太平洋螠虫成熟卵母细胞中的独特序列DNA转录本。

Unique sequence DNA transcripts present in mature oocytes of Urechis caupo.

作者信息

Davis F C

出版信息

Biochim Biophys Acta. 1975 Apr 16;390(1):33-45. doi: 10.1016/0005-2787(75)90006-4.

Abstract

DNA - DNA reannealing of sheared Urechis caupo DNA indicates that the genome is composed of two major repetitive sequence families. The repetitive sequence DNA families compose 8 and 7% of the DNA and are repeated in the genome on an average of 2.0 - 10-6 and 6.0 - 10-3 times, respectively. The unique sequence DNA composed a minimum of 75% of the genome and the rate of reannealing indicates a complexity of 2.6 - 10-8 nucleotide pairs per genome. Hybridization of isolated 3-H-labeled unique sequence DNA with an excess of unlabeled mature oocyte RNA provides a minimum estimate of the genetic diversity of the RNA present in the mature oocytes. The apparent saturation level of unique sequence DNA with RNA indicates that about 8.6% of the unique sequence DNA is expressed. Based on the fraction of the DNA that forms a stable hybrid and the complexity of the unique sequence DNA the RNA present in the mature oocyte is a complementary with 2.2 - 10-7 nucleotide pairs of unique sequence DNA.

摘要

对切碎的加州星虫DNA进行DNA - DNA复性分析表明，其基因组由两个主要的重复序列家族组成。这两个重复序列DNA家族分别占DNA的8%和7%，在基因组中的平均重复次数分别为2.0×10⁻⁶次和6.0×10⁻³次。单拷贝序列DNA至少占基因组的75%，复性速率表明每个基因组的复杂度为2.6×10⁻⁸核苷酸对。用过量的未标记成熟卵母细胞RNA与分离的³H标记单拷贝序列DNA进行杂交，可对成熟卵母细胞中存在的RNA的遗传多样性进行最低估计。单拷贝序列DNA与RNA的表观饱和水平表明，约8.6%的单拷贝序列DNA被表达。根据形成稳定杂交体的DNA比例和单拷贝序列DNA的复杂度，成熟卵母细胞中存在的RNA与2.2×10⁻⁷核苷酸对的单拷贝序列DNA互补。