莱茵衣藻叶绿体DNA的异质性、复杂性和重复性。
Heterogeneity, complexity, and repetition of the chloroplast DNA of Chlamydomonas reinhardtii.
作者信息
Bastia D, Chiang K S, Swift H, Siersma P
出版信息
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1157-61. doi: 10.1073/pnas.68.6.1157.
Abstract
The chloroplast DNA of wild-type Chlamydomonas reinhardtii was isolated in a CsCl density gradient as a single, homogeneous density class with a mean density of 1.695 g/cm(3). Irrespective of sheared size, denatured chloroplast DNA renatured as a single homogeneous species. Compositional heterogeneity, presumably intramolecular, was revealed by the absorbance-temperature profile. The complement of unique nucleotide sequences of the chloroplast DNA, as determined by the rate of renaturation, was 1.94 x 10(8) daltons. This kinetic complexity is 26-fold less than the DNA content of a single gamete chloroplast, and 52-fold less than the chloroplast of a vegetative cell, which indicates that the chloroplast of C. reinhardtii possesses at least 26 copies of a unique nucleotide sequence.
摘要
野生型莱茵衣藻的叶绿体DNA在氯化铯密度梯度中被分离出来，呈现为单一的均匀密度类，平均密度为1.695 g/cm³。无论剪切大小如何，变性的叶绿体DNA复性后均为单一的均匀物种。通过吸光度-温度曲线揭示了可能是分子内的组成异质性。根据复性速率确定，叶绿体DNA独特核苷酸序列的互补物为1.94×10⁸道尔顿。这种动力学复杂性比单个配子叶绿体的DNA含量少26倍，比营养细胞的叶绿体少52倍，这表明莱茵衣藻的叶绿体至少拥有26个独特核苷酸序列的拷贝。
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