利用大规模小鼠诱变筛选。
Capitalizing on large-scale mouse mutagenesis screens.
作者信息
Justice M J
机构信息
Department of Molecular and Human Genetics, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030, USA.
出版信息
Nat Rev Genet. 2000 Nov;1(2):109-15. doi: 10.1038/35038549.
Variation is the crux of genetics. Mutagenesis screens in organisms from bacteria to fish have provided a battery of mutants that define protein functions within complex pathways. Large-scale mutation isolation has been carried out in Caenorhabditis elegans, Drosophila melanogaster and zebrafish, and has been recently reported in the mouse in two screens that have generated many new, clinically relevant mutations to reveal the power of phenotype-driven screens in a mammal.
变异是遗传学的关键所在。从细菌到鱼类等生物体中的诱变筛选已提供了一系列突变体，这些突变体可定义复杂通路中的蛋白质功能。秀丽隐杆线虫、黑腹果蝇和斑马鱼都已开展了大规模的突变分离工作，最近在小鼠中也进行了两项筛选并报道了相关成果，这些筛选产生了许多新的、与临床相关的突变，以揭示在哺乳动物中表型驱动筛选的作用。
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