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60赫兹磁场对体外培养的人淋巴细胞的细胞学效应:姐妹染色单体交换、细胞动力学和有丝分裂率

Cytological effects of 60 Hz magnetic fields on human lymphocytes in vitro: sister-chromatid exchanges, cell kinetics and mitotic rate.

作者信息

Heredia-Rojas J A, Rodríguez-De La Fuente A O, del Roble Velazco-Campos M, Leal-Garza C H, Rodríguez-Flores L E, de La Fuente-Cortez B

机构信息

Subdirección de Postgrado, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, México.

出版信息

Bioelectromagnetics. 2001 Apr;22(3):145-9. doi: 10.1002/bem.32.

Abstract

Incubation for 72 h of human peripheral blood cultures in the presence of 60 Hz sinusoidal magnetic fields (MF) at magnetic flux densities of 1.0, 1.5, and 2.0 mT led to stimulation of lymphocyte proliferation but had no influence on the frequency of sister-chromatid exchanges (SCE). The cytotoxic potential of MF combined with the mutagen Mitomycin-C also was analyzed. An opposite effect between MF exposure and Mitomycin-C treatment in terms of cell kinetics and mitotic rate was found, whereas no variation in SCE frequency was observed for this coexposure condition.

摘要

在磁通密度为1.0、1.5和2.0 mT的60 Hz正弦磁场(MF)存在下,将人外周血培养物孵育72小时,可刺激淋巴细胞增殖,但对姐妹染色单体交换(SCE)频率没有影响。还分析了MF与诱变剂丝裂霉素-C联合使用时的细胞毒性潜力。在细胞动力学和有丝分裂率方面,发现MF暴露与丝裂霉素-C处理之间存在相反的效果,而在这种共同暴露条件下,未观察到SCE频率的变化。

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