携带Cre重组酶和显性营养缺陷型标记的质粒，适用于酿酒酵母和乳酸克鲁维酵母的原养型菌株。
Plasmids with the Cre-recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis.
作者信息
Steensma H Y, Ter Linde J J
机构信息
Institute for Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
出版信息
Yeast. 2001 Mar 30;18(5):469-72. doi: 10.1002/yea.696.
Two plasmids are described which can be used to remove the "loxP-markerMX-loxP" cassettes in strains lacking the ura3 mutation. Both contain the Cre-recombinase under control of the GAL1 promoter and the natMX cassette with the dominant marker nat, which gives yeasts resistance to the antibiotic ClonNat. pNatCre contains ARSH and CEN6 for maintenance in Saccharomyces cerevisiae. pKlNatCre has a Kluyveromyces lactis replication origin and centromere in addition.
本文描述了两种质粒，可用于在缺乏ura3突变的菌株中去除“loxP-标记MX-loxP”盒式结构。两者都含有在GAL1启动子控制下的Cre重组酶和带有显性标记nat的natMX盒式结构，该标记使酵母对抗生素ClonNat具有抗性。pNatCre包含用于在酿酒酵母中维持的ARSH和CEN6。pKlNatCre还具有乳酸克鲁维酵母复制起点和着丝粒。
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