Ribeiro Orquídea, Gombert Andreas K, Teixeira José A, Domingues Lucília
Institute of Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal.
J Biotechnol. 2007 Aug 1;131(1):20-6. doi: 10.1016/j.jbiotec.2007.05.027. Epub 2007 Jun 6.
The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a beta-galactosidase activity.
马克思克鲁维酵母具有几个有趣的特性，这些特性使该物种成为生产多种化合物的有前途的工业酵母。为了充分利用这种酵母及其特殊性质，需要合适的基因破坏和代谢工程工具。Cre-loxP系统是一种非常通用的工具，可实现基因标记拯救，从而产生不含外源选择标记的突变菌株，这对于工业应用来说是一个非常重要的方面。由于Cre-loxP系统在一些非传统酵母（即乳酸克鲁维酵母）中起作用，我们想知道它在马克思克鲁维酵母中是否也起作用。在这里，我们报告了通过破坏编码β-半乳糖苷酶活性的LAC4基因的两个拷贝，在马克思克鲁维酵母CBS 6556中对该系统的验证。
Zotero 插件