Zhao R, Li Y, Chen X
Institute of Reproductive Health, Beijing University, Beijing 100083, China.
Wei Sheng Yan Jiu. 2001 Jan;30(1):34-6.
In order to investigate the effect of homocysteine(HCY) on the development of rat embryos, the post-implantation whole embryo culture(WEC) technique was used. Neural plate stage(GD9.5) rat embryos were explanted in rat serum medium(immediately centrifugal serum, ICS) with D,L-HCY(0,0.15, 1.5,2.0,4.0,6.0,8.0,10.0 mmol/L), and cultured for 48 hours. The results showed that HCY did cause damage to embryonic development and the damages of HCY on embryos were characterized in a significant dose-response pattern. The minimum teratogenic dose of D,L-HCY was 0.15 mmol/L, the incidence of dysmorphogenic embryos was 8.33%. Significant inhibiting effects of HCY on yolk sac(including reduced yolk sac diameter, shriveled surface, small or defective yolk sac vessels) and embryonic growth and morpholological differentiation were apparent with increased HCY, while HCY were 4 mmol/L or more (P < 0.05). The abnormalities included neural tube defects, delayed cardiac tube formation, pericardial effusion, incomplete flexion, small or missing forelimb buds and irregular somites, etc. These findings suggested that HCY might exert a direct effect on embryos and a "double effect" both on yolk sac and embryos.
为了研究同型半胱氨酸（HCY）对大鼠胚胎发育的影响，采用植入后全胚胎培养（WEC）技术。将神经板期（GD9.5）大鼠胚胎植入含有D，L - HCY（0、0.15、1.5、2.0、4.0、6.0、8.0、10.0 mmol/L）的大鼠血清培养基（立即离心血清，ICS）中，培养48小时。结果表明，HCY确实对胚胎发育造成损害，且HCY对胚胎的损害呈现出显著的剂量反应模式。D，L - HCY的最小致畸剂量为0.15 mmol/L，畸形胚胎的发生率为8.33%。随着HCY浓度增加，HCY对卵黄囊（包括卵黄囊直径减小、表面皱缩、卵黄囊血管细小或有缺陷）以及胚胎生长和形态分化有明显的抑制作用，当HCY浓度达到4 mmol/L及以上时（P < 0.05）。异常情况包括神经管缺陷、心脏管形成延迟、心包积液、屈曲不全、前肢芽小或缺失以及体节不规则等。这些发现表明，HCY可能对胚胎产生直接影响，并且对卵黄囊和胚胎都有“双重作用”。
