Qu W, Zhang B, Wu D, Wu W
Department of Environmental Health, School of Public Health, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2000 Jan 30;29(1):17-9.
In order to explore the effects of drinking alcohol during pregnancy on embryonic development and its mechanisms, a post-implantation whole embryo culture(WEC) technique was used. The 9.5 day rat embryos were explanted in rat serum medium(immediately centrifugal serum, ICS) with alcohol(0.0.4.1.0, 2.00 and 4.00 g/L), and cultured for 48 hours. The index of embryo development and morphological scores induced by alcohol were observed. The result showed that alcohol had obviously effects on the development and growth of embryos with a dose-response relationship. Embryonic development of 0.4 g/L group was not significantly different from the control group, whereas 1.0 g/L group could interfere with the development score of mid-brain, forebrain, neurotube, and visceral yolk sac(VYS) circle obviously. All scores of the 2.00 g/L group were significantly lower than that of control group (P < 0.05). Moreover, the rate of embryo lethality and teratogenecity were obvious increased. It is concluded that alcohol has developmental toxicity and teratogenicity. The target organ affected by alcohol is brain. The effects of alcohol on the developmental differentiation of visceral yolk sac and DNA synthesis are probably related to its developmental abnormalities.
为探讨孕期饮酒对胚胎发育的影响及其机制，采用植入后全胚胎培养（WEC）技术。将9.5天龄的大鼠胚胎植入含酒精（0.0、0.4、1.0、2.00和4.00 g/L）的大鼠血清培养基（立即离心血清，ICS）中，培养48小时。观察酒精诱导的胚胎发育指标和形态学评分。结果表明，酒精对胚胎的发育和生长有明显影响，且呈剂量反应关系。0.4 g/L组胚胎发育与对照组无显著差异，而1.0 g/L组可明显干扰中脑、前脑、神经管和内脏卵黄囊（VYS）环的发育评分。2.00 g/L组的所有评分均显著低于对照组（P < 0.05）。此外，胚胎致死率和致畸率明显增加。结论是酒精具有发育毒性和致畸性。酒精影响的靶器官是脑。酒精对内脏卵黄囊发育分化和DNA合成的影响可能与其发育异常有关。
