Farrell A M, Dean D, Charnock M, Wojnarowska F
Departments of Dermatology and of Obstetrics and Gynaecology, Oxford Radcliffe Hospitals, Oxford University, Oxford, U.K.
J Reprod Med. 2001 Feb;46(2):117-24.
To study the distribution of transforming growth factor beta (TGF-beta) isoforms TGF-beta 1, TGF-beta 2 and TGF-beta 3 and vascular endothelial growth factor (VEGF) in vulvar lichen sclerosus.
Biopsies were obtained from 10 patients with vulvar lichen sclerosus, snap frozen and stained with polyclonal antibodies to TGF-beta 1, TGF-beta 2, TGF-beta 3 and VEGF. Control tissues used were uninvolved thigh tissue from two of the lichen sclerosus patients and normal vulvar tissue obtained from eight patients during gynecologic procedures. Two specimens of morphea were also examined.
Weak TGF-beta 1 staining was demonstrated in the epidermis of all the lichen sclerosus, morphea, thigh and five of the eight normal vulvar specimens. Slight increase in TGF-beta 1 staining was seen in the upper and middermis in 6 of the 10 lichen sclerosus specimens and in the morphea specimens as compared to the control tissue, and this staining was localized within cells. TGF-beta 2 staining was present throughout the epidermis in all the normal thigh, normal vulva, lichen sclerosus and morphea specimens. TGF-beta 2 staining was increased within cells in the upper and middermis of the lichen sclerosus and morphea specimens. TGF-beta 3 staining occurred in the basal half of the epidermis of all the control, lichen sclerosus and morphea specimens, and only slight upper dermal staining of a few individual cells was seen in 3 of the 10 lichen sclerosus specimens. VEGF staining was similar in the normal tissues, lichen sclerosus and morphea.
These results suggest that TGF-beta may.
研究转化生长因子β（TGF-β）亚型TGF-β1、TGF-β2和TGF-β3以及血管内皮生长因子（VEGF）在外阴硬化性苔藓中的分布。
从10例外阴硬化性苔藓患者获取活检组织，速冻后用抗TGF-β1、TGF-β2、TGF-β3和VEGF的多克隆抗体进行染色。所用对照组织为2例硬化性苔藓患者未受累的大腿组织以及8例患者在妇科手术中获取的正常外阴组织。还检查了2例硬斑病标本。
在所有硬化性苔藓、硬斑病、大腿组织以及8例正常外阴标本中的5例的表皮中均显示出TGF-β1弱阳性染色。与对照组织相比，在10例硬化性苔藓标本中的6例以及硬斑病标本的上、中层真皮中可见TGF-β1染色略有增加，且这种染色定位于细胞内。在所有正常大腿、正常外阴、硬化性苔藓和硬斑病标本的整个表皮中均存在TGF-β2染色。在硬化性苔藓和硬斑病标本的上、中层真皮细胞内TGF-β2染色增加。在所有对照、硬化性苔藓和硬斑病标本的表皮基底层均出现TGF-β3染色，在10例硬化性苔藓标本中的3例仅见少数单个细胞的真皮浅层轻微染色。正常组织、硬化性苔藓和硬斑病中的VEGF染色相似。
这些结果提示TGF-β可能……
