Mdt(A)，一种在乳酸乳球菌和大肠杆菌中赋予多重抗生素抗性的新型外排蛋白。
Mdt(A), a new efflux protein conferring multiple antibiotic resistance in Lactococcus lactis and Escherichia coli.
作者信息
Perreten V, Schwarz F V, Teuber M, Levy S B
机构信息
Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
出版信息
Antimicrob Agents Chemother. 2001 Apr;45(4):1109-14. doi: 10.1128/AAC.45.4.1109-1114.2001.
Abstract
The mdt(A) gene, previously designated mef214, from Lactococcus lactis subsp. lactis plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned mdt(A) gene decreased susceptibility to macrolides, lincosamides, streptogramins, and tetracyclines in L. lactis and Escherichia coli, but not in Enterococcus faecalis or in Staphylococcus aureus. Glucose-dependent efflux of erythromycin and tetracycline was demonstrated in L. lactis and in E. coli.
摘要
来自乳酸乳球菌乳亚种质粒pK214的mdt(A)基因，先前被命名为mef214，编码一种具有12个推定跨膜区段（TMS）的蛋白质[Mdt(A)（多药转运蛋白）]，这些区段包含主要转运体超家族外排蛋白中保守的典型基序。然而，它也有两个C基序（在反向转运体的第五个TMS中保守）和一个推定的ATP结合位点。克隆的mdt(A)基因的表达降低了乳酸乳球菌和大肠杆菌对大环内酯类、林可酰胺类、链阳菌素类和四环素类药物的敏感性，但对粪肠球菌或金黄色葡萄球菌没有影响。在乳酸乳球菌和大肠杆菌中证实了葡萄糖依赖性的红霉素和四环素外排。
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