一个赋予异源宿主大肠杆菌多药耐药性的谷氨酸棒杆菌基因。
A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.
作者信息
Jäger W, Kalinowski J, Pühler A
机构信息
Lehrstuhl für Genetik, Universität Bielefeld, Germany.
出版信息
J Bacteriol. 1997 Apr;179(7):2449-51. doi: 10.1128/jb.179.7.2449-2451.1997.
Abstract
A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.
摘要
来自对红霉素敏感的谷氨酸棒状杆菌ATCC 13032的一个染色体DNA片段被证明可介导大肠杆菌对红霉素、四环素、嘌呤霉素和博来霉素产生抗性。该片段的多拷贝克隆在谷氨酸棒状杆菌中未产生抗性表型。相应的基因编码一种具有12个潜在跨膜α螺旋片段的疏水蛋白，该蛋白与药物-H⁺反向转运体具有相似性。
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