Accardi Luisa, Prehaud Christophe, Di Bonito Paola, Mochi Stefania, Bouloy Michèle, Giorgi Colomba
Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy1.
Groupes des Bunyaviridés, Unité des Arbovirus et virus des Fièvres Haemorragiques, Institut Pasteur, 75724 Paris Cedex, France2.
J Gen Virol. 2001 Apr;82(Pt 4):781-785. doi: 10.1099/0022-1317-82-4-781.
A transcription system for Toscana virus (TOSV) (a member of the family BUNYAVIRIDAE:, genus PHLEBOVIRUS:) was constructed. For in vivo expression, the TOSV transcription system uses the viral N and L proteins and an S-like RNA genome containing the chloramphenicol acetyltransferase reporter gene in the antisense orientation flanked by the viral genomic 5'- and 3'-terminal S sequences. It was found that the N and L proteins represent the minimal protein requirement for an active transcription complex. To investigate the possibility of reassortment between TOSV and Rift Valley fever virus (RVFV), the activity of their polymerase complexes was tested on their heterologous S-like RNA genomes and this showed that both virus complexes were active. Moreover, hybrid transcriptase complexes with protein components originating from the two viruses were tested on both virus templates and only the combination RVFV L + TOSV N on RVFV S-like RNA was found to be active in this assay. These results suggest that virus reassortants might be generated whenever the two viruses infect the same host.
构建了托斯卡纳病毒（TOSV）（布尼亚病毒科白蛉病毒属的成员）的转录系统。为了在体内表达，TOSV转录系统使用病毒N蛋白和L蛋白以及一个类似S的RNA基因组，该基因组在反义方向上含有氯霉素乙酰转移酶报告基因，两侧为病毒基因组5'和3'末端的S序列。发现N蛋白和L蛋白是活性转录复合体所需的最小蛋白质。为了研究TOSV与裂谷热病毒（RVFV）之间重配的可能性，在它们的异源类似S的RNA基因组上测试了它们聚合酶复合体的活性，结果表明两种病毒复合体均具有活性。此外，在两种病毒模板上测试了含有源自两种病毒的蛋白质成分的杂交转录酶复合体，在该试验中仅发现RVFV L + TOSV N组合在RVFV类似S的RNA上具有活性。这些结果表明，只要两种病毒感染同一宿主，就可能产生病毒重配体。
