Weidmann Manfred, Sanchez-Seco M Paz, Sall Amadou A, Ly Peinda Ogo, Thiongane Yaya, Lô Modou M, Schley Heike, Hufert Frank T
Institute of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany.
J Clin Virol. 2008 Feb;41(2):138-42. doi: 10.1016/j.jcv.2007.10.001. Epub 2007 Nov 19.
Rapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae.
To develop RT-PCR assays for Sandfly Fever Sicilian virus (SFSV), Sandfly Fever Naples virus (SFNV), Toscana virus (TOSV) and Rift Valley Fever virus (RVFV).
RNA standards were generated and used to test the performance of the assays.
A detection limit of 10-100 RNA molecules was determined for the SFSV, TOSV and RVFV assays. The sensitivity of the SFNV assay was not determined. The TOSV and the RVFV assays detected recent isolates from Spain and Africa, respectively.
The assays should help to improve surveillance of pathogenic Phleboviruses.
对于布尼亚病毒科白蛉病毒属中的几种人类病原体，目前尚无快速诊断方法。
开发用于西西里白蛉热病毒（SFSV）、那不勒斯白蛉热病毒（SFNV）、托斯卡纳病毒（TOSV）和裂谷热病毒（RVFV）的逆转录聚合酶链反应（RT-PCR）检测方法。
生成RNA标准品并用于测试检测方法的性能。
确定了SFSV、TOSV和RVFV检测方法的检测限为10 - 100个RNA分子。未确定SFNV检测方法的灵敏度。TOSV和RVFV检测方法分别检测到来自西班牙和非洲的近期分离株。
这些检测方法应有助于改善对致病性白蛉病毒的监测。
