Saravolac E G, Sabuda D, Crist C, Blasetti K, Schnell G, Yang H, Kende M, Levy H B, Wong J P
Chemical and Biological Defence Section, Defence Research Establishment Suffield, Box 4000 Station Main, Alberta, T1A 8K6, Medicine Hat, Canada.
Vaccine. 2001 Mar 21;19(17-19):2227-32. doi: 10.1016/s0264-410x(00)00450-3.
The objective of this report is to evaluate the prophylactic efficacy of liposome-mediated immunotherapy for prevention of respiratory influenza virus infection in mice. Antiviral antibody, interferon-gamma and poly (ICLC) were encapsulated in liposomes and they were evaluated for their ability to induce protective immunity against lethal influenza infection. Passive immunization using liposome-encapsulated antiviral antibody was found to offer complete protection against the virus challenge. However, this pretreatment must be administered within 24 h prior to virus challenge to be protective. Pretreatment with liposome-encapsulated interferon-gamma was found to stimulate cellular immune responses, but the protection is partial. Immunoprophylaxis using liposome-encapsulated double-stranded (ds) RNA poly (ICLC) provided complete and longer-lasting protection against influenza infection. These results suggest liposome-mediated immunoprophylactic approaches are effective in the prevention of respiratory influenza virus infection.
本报告的目的是评估脂质体介导的免疫疗法对预防小鼠呼吸道流感病毒感染的预防效果。抗病毒抗体、γ干扰素和聚肌胞苷酸（聚肌苷酸-聚胞嘧啶核苷酸）被包裹在脂质体中，并对它们诱导针对致死性流感感染的保护性免疫的能力进行了评估。发现使用脂质体包裹的抗病毒抗体进行被动免疫可提供针对病毒攻击的完全保护。然而，这种预处理必须在病毒攻击前24小时内进行才能起到保护作用。发现用脂质体包裹的γ干扰素进行预处理可刺激细胞免疫反应，但保护作用是部分的。使用脂质体包裹的双链（ds）RNA聚肌胞苷酸（聚肌苷酸-聚胞嘧啶核苷酸）进行免疫预防可提供针对流感感染的完全且持久的保护。这些结果表明脂质体介导的免疫预防方法在预防呼吸道流感病毒感染方面是有效的。
