Cáceda R, Gamboa J L, Boero J A, Monge-C C, Arregui A
Laboratorio de Neurociencias, Universidad Peruana Cayetano Heredia, Lima, Peru.
Neurosci Lett. 2001 Apr 6;301(3):171-4. doi: 10.1016/s0304-3940(01)01630-5.
We measured the activities of Na(+)K(+) ATPase and of enzymes of the glycolytic pathway, Krebs cycle, and the respiratory chain in cerebral cortex of mice exposed to chronic hypoxia for three weeks and compared their values with those of sea level controls. There were no differences in Na(+)K(+) ATPase activity or in the activity of glycolytic enzymes. In the Krebs cycle, a 66% increase of succinate dehydrogenase activity was found due to a lower Km. In contrast, respiratory chain cytochrome oxidase activity was reduced by 12% in mice exposed to hypoxia. This suggested that the metabolic demand would be satisfied despite the respiratory chain depression (cytochrome oxidase), probably due to anaerobic energy production within the mitochondria (succinate dehydrogenase).
我们测量了暴露于慢性缺氧环境三周的小鼠大脑皮层中钠钾ATP酶以及糖酵解途径、三羧酸循环和呼吸链中各种酶的活性，并将其值与海平面对照组进行了比较。钠钾ATP酶活性或糖酵解酶活性没有差异。在三羧酸循环中，由于较低的米氏常数，琥珀酸脱氢酶活性增加了66%。相比之下，暴露于缺氧环境的小鼠呼吸链细胞色素氧化酶活性降低了12%。这表明，尽管呼吸链（细胞色素氧化酶）受到抑制，但代谢需求可能仍能得到满足，这可能是由于线粒体内的无氧能量产生（琥珀酸脱氢酶）所致。
