多变鱼腥藻ATCC 29413缺乏藻青素合成酶的突变体：生长特性和超微结构方面
A mutant of the cyanobacterium Anabaena variabilis ATCC 29413 lacking cyanophycin synthetase: growth properties and ultrastructural aspects.
作者信息
Ziegler K, Stephan D P, Pistorius E K, Ruppel H G, Lockau W
机构信息
Institut für Biologie, Biochemie der Pflanzen, Humboldt-Universität zu Berlin, Germany.
出版信息
FEMS Microbiol Lett. 2001 Mar 1;196(1):13-8. doi: 10.1111/j.1574-6968.2001.tb10533.x.
The gene cphA encoding cyanophycin synthetase was interrupted in Anabaena variabilis ATCC 29413 by insertional mutagenesis. The mutant lacked cyanophycin granules and the polar nodules of heterocysts. The mutant grew as fast as the wild-type irrespective of the nitrogen source at low light intensity whereas growth on N(2) was somewhat reduced in high light. It is concluded that cyanophycin metabolism and polar nodules are not essential for aerobic N(2) fixation.
通过插入诱变，编码藻青素合成酶的基因cphA在多变鱼腥藻ATCC 29413中被中断。该突变体缺乏藻青素颗粒和异形胞的极节。无论氮源如何，该突变体在低光照强度下的生长速度与野生型一样快，而在高光下，其在N₂上的生长有所减少。得出的结论是，藻青素代谢和极节对于好氧性N₂固定不是必需的。
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