多变鱼腥藻中第二种钼依赖型固氮酶基因的特性分析
Characterization of genes for a second Mo-dependent nitrogenase in the cyanobacterium Anabaena variabilis.
作者信息
Thiel T, Lyons E M, Erker J C
机构信息
Department of Biology, University of Missouri-St. Louis, 63121, USA.
出版信息
J Bacteriol. 1997 Aug;179(16):5222-5. doi: 10.1128/jb.179.16.5222-5225.1997.
Abstract
Anabaena variabilis ATCC 29413 is a filamentous heterocystous cyanobacterium that fixes nitrogen under a variety of environmental conditions. Under aerobic growth conditions, nitrogen fixation depends upon differentiation of heterocysts and expression of either a Mo-dependent nitrogenase or a V-dependent nitrogenase in those specialized cells. Under anaerobic conditions, a second Mo-dependent nitrogenase gene cluster, nifII, was expressed in vegetative cells long before heterocysts formed. A strain carrying a mutant gene in the nifII cluster did not fix nitrogen under anaerobic conditions until after heterocysts differentiated. The nifII cluster was similar in organization to the nifI cluster that is expressed in heterocysts and that includes nifBSUHDKENXW as well as three open reading frames that are conserved in both cyanobacterial nif clusters.
摘要
多变鱼腥藻ATCC 29413是一种丝状、具异形胞的蓝细菌，能在多种环境条件下固氮。在有氧生长条件下，固氮依赖于异形胞的分化以及这些特化细胞中钼依赖型固氮酶或钒依赖型固氮酶的表达。在厌氧条件下，第二个钼依赖型固氮酶基因簇nifII在异形胞形成之前很久就在营养细胞中表达。携带nifII簇中突变基因的菌株在厌氧条件下直到异形胞分化后才固氮。nifII簇在组织上与在异形胞中表达的nifI簇相似，nifI簇包括nifBSUHDKENXW以及在两个蓝细菌固氮基因簇中都保守的三个开放阅读框。
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Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required when iron is limited.蓝藻鱼腥藻在分子态氮上的生长：当铁受到限制时需要固氮酶J。
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Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142.蓝细菌蓝丝菌属ATCC 51142菌株中碳水化合物颗粒形成和固氮的振荡行为。
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