天蓝色链霉菌麦芽糖糊精ABC转运蛋白的合成取决于调节因子MalR的存在。
Synthesis of the Streptomyces lividans maltodextrin ABC transporter depends on the presence of the regulator MalR.
作者信息
Schlösser A, Weber A, Schrempf H
机构信息
FB Biologie/Chemie, Universität Osnabrück, Barbarastrasse 11, D-49069, Osnabrück, Germany
出版信息
FEMS Microbiol Lett. 2001 Mar 1;196(1):77-83. doi: 10.1111/j.1574-6968.2001.tb10544.x.
During growth with maltotriose or amylose, Streptomyces lividans and Streptomyces coelicolor A3(2) synthesize a maltodextrin uptake system with highest specificity for maltotriose. The transport activity is absent in mutants of S. coelicolor A3(2) lacking a functional MalE binding protein. Cloning and sequencing data suggest that the mal operon of S. coelicolor A3(2) corresponds to the one of S. lividans and that the deduced S. lividans Reg1 amino acid sequence is identical to that of MalR from S. coelicolor A3(2). It can be concluded that both strains have the same ABC transport system for maltodextrins. The S. lividans malR was cloned in Escherichia coli in frame with six histidine-encoding codons. The resulting, purified 6HisMalR(SI) was shown to bind to two motifs within the S. lividans malR-malE intergenic region and to dissociate in the presence of maltopentaose.
在以麦芽三糖或直链淀粉为碳源生长时，变铅青链霉菌和天蓝色链霉菌A3(2)会合成一种对麦芽三糖具有最高特异性的麦芽糊精摄取系统。在缺乏功能性MalE结合蛋白的天蓝色链霉菌A3(2)突变体中不存在转运活性。克隆和测序数据表明，天蓝色链霉菌A3(2)的mal操纵子与变铅青链霉菌的mal操纵子相对应，并且推导的变铅青链霉菌Reg1氨基酸序列与天蓝色链霉菌A3(2)的MalR氨基酸序列相同。可以得出结论，这两种菌株具有相同的麦芽糊精ABC转运系统。变铅青链霉菌malR基因与六个组氨酸编码密码子框内克隆到大肠杆菌中。所得纯化的6HisMalR(SI)显示与变铅青链霉菌malR-malE基因间区域内的两个基序结合，并在麦芽五糖存在下解离。
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