Young R A, Smith R E
Can J Microbiol. 1975 May;21(5):587-91. doi: 10.1139/m75-085.
Methionine-excreting mutant strains of Streptomyces fradiae ATCC 10745, producedby exposure to N-methyl-N'-nitro-N-nitrosoguanidine, were selected on the basisof ethionine-resistance and ability to support the growth of an auxotroph which requiredmethionine or methionine precursors. When acid hydrolysates of dehydrated liquid cultures were analyzed for amino acid content, one of the mutants had produced 268% more methionine that the parental strain. To obtain an accurate analysis of methionine content, it was necessary to determine the amount of diaminopimelic acid which had been synthesized. This compound appeared in eluates during column chromatography at about the same time as methionine, and its presence increased apparent methionine concentrations by 25 to40%.
通过用N-甲基-N'-硝基-N-亚硝基胍处理得到的弗氏链霉菌ATCC 10745的蛋氨酸分泌突变菌株，是根据对乙硫氨酸的抗性以及支持需要蛋氨酸或蛋氨酸前体的营养缺陷型生长的能力来筛选的。当分析脱水液体培养物的酸水解产物中的氨基酸含量时，其中一个突变体产生的蛋氨酸比亲本菌株多268%。为了准确分析蛋氨酸含量，有必要确定已合成的二氨基庚二酸的量。该化合物在柱色谱洗脱液中出现的时间与蛋氨酸大致相同，其存在使表观蛋氨酸浓度增加了25%至40%。
