Hagedorn C, Holt J G
Can J Microbiol. 1975 May;21(5):688-93. doi: 10.1139/m75-099.
Twenty-five gram-negative and 62 gram-positive bacterial cultures, of which 37 werearthrobacters, were tested, using a multipoint inoculation device, for responses on varying concentrations of 35 dyes. Both selective (growth vs. no growth) and differential (dye absorption) responses were obtained. It was possible to differentiate the arthrobacters from the other gram-positive bacteria tested as well as to distinguish separately each Arthrobacter-named strain. The results indicated that dyes have a greater potential for use in selective and differential media than has been presently realized; and the possibility of using dye-reactions as features in taxonomic schemes to differentiate rapidly many of the common genera of soil bacteria is discussed.
使用多点接种装置,对25株革兰氏阴性菌培养物和62株革兰氏阳性菌培养物(其中37株为节杆菌)进行了测试,观察它们对35种不同浓度染料的反应。获得了选择性反应(生长与不生长)和鉴别性反应(染料吸收)。能够将节杆菌与所测试的其他革兰氏阳性菌区分开来,也能分别区分每个命名为节杆菌的菌株。结果表明,染料在选择性和鉴别性培养基中的应用潜力比目前所认识到的更大;并讨论了将染料反应作为分类学方案中的特征来快速区分许多常见土壤细菌属的可能性。