Sieburth J M
J Bacteriol. 1967 Jun;93(6):1911-6. doi: 10.1128/jb.93.6.1911-1916.1967.
The bacterial flora of water in Narragansett Bay, R.I., was observed semimonthly from 1962 to 1964. Dominant isolates were keyed to genus, and the isolates for each genus were expressed as percentage of total isolates. There was a consistent inverse relationship between arthrobacters and the dominant pseudomonads. Pseudomonad growth on agar plates markedly inhibited arthrobacter cross-streaks. Agar from inhibition zones as well as supernatant fluids from pseudomonad broth cultures inhibited arthrobacter motility and caused the cells to agglutinate. Gummy pseudomonad residues from vacuum-evaporated broth cultures readily passed a G-25 Sephadex column. This material agglutinated arthrobacter cells, but failed to cause arthrobacter inhibition in filter-pad assays. In contrast, sterile medium inside a dialysis sac, inoculated externally with a pseudomonad, was inhibitory to arthrobacters in pad assay but failed to agglutinate arthrobacter cells. Pseudomonad isolates from soil showed similar inhibiting and agglutinating activities for both soil and seawater arthrobacter isolates. The inhibitory and agglutinating activities of pseudomonad isolates appeared to diminish on prolonged laboratory cultivation.
1962年至1964年期间，对罗德岛纳拉甘西特湾水体中的细菌菌群进行了半月一次的观测。将优势分离株鉴定到属，并将每个属的分离株表示为总分离株的百分比。节杆菌与优势假单胞菌之间存在一致的负相关关系。假单胞菌在琼脂平板上的生长显著抑制节杆菌的交叉划线。来自抑制区的琼脂以及假单胞菌肉汤培养物的上清液抑制节杆菌的运动性并导致细胞凝集。真空蒸发肉汤培养物中的粘性假单胞菌残留物很容易通过G - 25葡聚糖凝胶柱。这种物质使节杆菌细胞凝集，但在滤垫试验中未能导致节杆菌受到抑制。相反，在透析袋内的无菌培养基，外部接种假单胞菌后，在垫试验中对节杆菌有抑制作用，但未能使节杆菌细胞凝集。从土壤中分离得到的假单胞菌对土壤和海水节杆菌分离株均表现出类似的抑制和凝集活性。假单胞菌分离株的抑制和凝集活性在长期实验室培养后似乎会减弱。
