Young E, Kajander K C, Rimell F, Ho B, Goldman J
Department of Otolaryngology, Head and Neck Surgery, University of Minnesota, Minneapolis, USA.
Am J Rhinol. 2001 Jan-Feb;15(1):61-7. doi: 10.2500/105065801781329374.
Substance P (SP), one of the neuropeptides released from sensory nerves, is thought to mediate neurogenic inflammation. Although SP immunoreactive axons have been described in the sinus mucosa, no attempt has been made to characterize SP fibers as a subset of all axons present in the sinus mucosa. In addition, no study to date has characterized the changes in infected sinus mucosa. The maxillary sinus mucosa of New Zealand white rabbits was harvested from control animals and in animals with induced maxillary sinusitis. Immunohistochemical staining of the sinus mucosa for both Protein Gene Product 9.5 (PGP), a nonspecific marker for all nerves, and for SP was performed on 11 animals: 3 controls and 8 infected. In sinus mucosa from the control rabbits, <50% of all axons labeled by PGP were immunoreactive for SP. In infected mucosa, the absolute number of axons found by PGP staining decreased and nearly all of these remaining fibers were also immunoreactive for SP. We conclude that the phenotypical labeling of nerve fibers seen in normal mucosa is altered by bacterial-induced infection.
P物质（SP）是感觉神经释放的神经肽之一，被认为可介导神经源性炎症。尽管已在鼻窦黏膜中描述了SP免疫反应性轴突，但尚未尝试将SP纤维表征为鼻窦黏膜中所有轴突的一个子集。此外，迄今为止尚无研究对感染的鼻窦黏膜的变化进行表征。从对照动物和诱发上颌窦炎的动物中采集新西兰白兔的上颌窦黏膜。对11只动物（3只对照动物和8只感染动物）的鼻窦黏膜进行了免疫组织化学染色，分别检测了所有神经的非特异性标志物蛋白基因产物9.5（PGP）以及SP。在对照兔的鼻窦黏膜中，PGP标记的所有轴突中<50%对SP呈免疫反应性。在感染的黏膜中，PGP染色发现的轴突绝对数量减少，几乎所有这些剩余纤维也对SP呈免疫反应性。我们得出结论，细菌感染会改变正常黏膜中所见神经纤维的表型标记。
