多药耐药蛋白1（MRP1）在大鼠耳蜗中的表达及其与血-内耳屏障的关系
Expression of multidrug resistance protein 1 (MRP1) in the rat cochlea with special reference to the blood-inner ear barrier.
作者信息
Saito T, Zhang Z J, Tokuriki M, Ohtsubo T, Noda I, Shibamori Y, Yamamoto T, Saito H
机构信息
Department of Otolaryngology, Fukui Medical University, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan.
出版信息
Brain Res. 2001 Mar 23;895(1-2):253-7. doi: 10.1016/s0006-8993(01)02020-0.
Expression of multidrug resistance protein 1 (MRP1) was detected in the rat cochlea by RT-PCR and immunohistochemistry using anti-MRP monoclonal antibody MRPr1. Use of primers specific for rat mrp1 gene resulted in the amplification of an expected 394 bp fragment prepared from brain and cochlear tissues. Immunohistochemically, MRP was found in the choroid plexus, stria vascularis, spiral ligament, spiral prominence and cochlear nerve in the modiolus. From these results, it was suggested that MRP in the rat cochlea might function as an extrusion pump and play an important role in the blood-inner ear barrier.
采用逆转录聚合酶链反应（RT-PCR）以及使用抗多药耐药蛋白1（MRP1）单克隆抗体MRPr1的免疫组织化学方法，在大鼠耳蜗中检测MRP1的表达。使用针对大鼠mrp1基因的特异性引物，可从脑和耳蜗组织中扩增出预期的394 bp片段。免疫组织化学结果显示，在脉络丛、血管纹、螺旋韧带、螺旋凸以及蜗轴内的耳蜗神经中发现了MRP。根据这些结果推测，大鼠耳蜗中的MRP可能作为一种外排泵发挥作用，并在血-内耳屏障中起重要作用。
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