Shamardin V A, Tsarevskil Y P, Karalnik B V
J Hyg Epidemiol Microbiol Immunol. 1976;21(3):353-60.
The effect of ionic parameters of the medium (pH and ionic strength) on the processes of interaction of tannin-treated erythrocytes and the protein fractions of human serum (macroglobulins, microbulins and albumin) was studied in factorial experiments. Complex effect of these parametres on the processes under investigation and optimum conditions of erythrocyte sensitization were established. Subsequent fixation of antibodies by the erythrocyte diagnostic and their agglutinating activity are manifested in different mannera depending on the conditions of preceding sensitization. Important peculairities were discovered in the mechanism of interaction between the erythrocytes and various serum proteins. The obtained results should be taken into account in the production of erythrocyte antigen and antibody diagnosticums.
在析因实验中研究了介质的离子参数（pH值和离子强度）对经单宁处理的红细胞与人血清蛋白组分（巨球蛋白、微球蛋白和白蛋白）相互作用过程的影响。确定了这些参数对所研究过程的综合影响以及红细胞致敏的最佳条件。红细胞诊断中抗体的后续固定及其凝集活性根据先前致敏的条件以不同方式表现出来。在红细胞与各种血清蛋白的相互作用机制中发现了重要特性。在生产红细胞抗原和抗体诊断试剂时应考虑所获得的结果。
