Hicks J, Lockington R A, Strauss J, Dieringer D, Kubicek C P, Kelly J, Keller N
Department of Plant Pathology and Microbiology, TAMUS 2132, Texas A&M University, College Station, TX 77843-2132, USA.
Mol Microbiol. 2001 Mar;39(6):1482-93. doi: 10.1046/j.1365-2958.2001.02332.x.
Aspergillus nidulans rcoA encodes a member of the WD repeat family of proteins. The RcoA protein shares sequence similarity with other members of this protein family, including the Saccharomyces cerevisiae Tup1p and Neurospora crassa RCO1. Tup1p is involved in negative regulation of an array of functions including carbon catabolite repression. RCO1 functions in regulating pleiotropic developmental processes, but not carbon catabolite repression. In A. nidulans, deletion of rcoA (DeltarcoA), a recessive mutation, resulted in gross defects in vegetative growth, asexual spore production and sterigmatocystin (ST) biosynthesis. Expression of the asexual and ST pathway-specific regulatory genes, brlA and aflR, respectively, but not the signal transduction genes (i.e. flbA, fluG or fadA) regulating brlA and aflR expression was delayed (brlA) or eliminated (aflR) in a DeltarcoA strain. Overexpression of aflR in a DeltarcoA strain could not rescue normal expression of downstream targets of AflR. CreA-dependent carbon catabolite repression of starch and ethanol utilization was only weakly affected in a DeltarcoA strain. The strong role of RcoA in development, vegetative growth and ST production, compared with a relatively weak role in carbon catabolite repression, is similar to the role of RCO1 in N. crassa.
构巢曲霉rcoA编码WD重复蛋白家族的一个成员。RcoA蛋白与该蛋白家族的其他成员具有序列相似性，包括酿酒酵母Tup1p和粗糙脉孢菌RCO1。Tup1p参与一系列功能的负调控，包括碳代谢物阻遏。RCO1在调节多效性发育过程中起作用，但不参与碳代谢物阻遏。在构巢曲霉中，rcoA的缺失（ΔrcoA），一种隐性突变，导致营养生长、无性孢子产生和柄曲霉素（ST）生物合成出现严重缺陷。在ΔrcoA菌株中，无性和ST途径特异性调控基因brlA和aflR的表达分别延迟（brlA）或消除（aflR），但调节brlA和aflR表达的信号转导基因（即flbA、fluG或fadA）不受影响。在ΔrcoA菌株中过表达aflR不能挽救AflR下游靶点的正常表达。在ΔrcoA菌株中，CreA依赖的淀粉和乙醇利用的碳代谢物阻遏仅受到微弱影响。与在碳代谢物阻遏中相对较弱的作用相比，RcoA在发育、营养生长和ST产生中的重要作用与粗糙脉孢菌中RCO1的作用相似。
