Psenicnik M, Veranic P
Institute of Cell Biology, Medical Faculty, University of Ljubljana, Slovenia.
Folia Histochem Cytobiol. 2001;39(1):3-8.
The mouse gallbladder epithelial cells contain very heterogeneous vacuolar population. In an attempt to classify these vacuoles we identified NADPase and TPPase activity as well as the location of HRP which is used as the endocytotic marker. The results of the present study show that the vacuoles can be classified into three categories: (1) the vacuoles predominantly containing loose membrane coils related to the nascent autophagic vacuoles, (2) vacuoles containing densely packed membranes and exhibiting a positive HRP reaction, indicating the convergence of endocytotic and autophagic pathway, and (3) vacuoles composed of degraded membrane structures and containing the reaction product of NADPase activity, showing that the fusion of the lysosomes with the autophagosome-endosome took place. The highly developed cis, medial and trans Golgi compartments reflect the biosynthetic and endocytotic activity of the gallbladder epithelium.
小鼠胆囊上皮细胞含有非常异质的液泡群体。为了对这些液泡进行分类，我们鉴定了NADP酶和TPP酶活性以及用作内吞标记物的辣根过氧化物酶（HRP）的定位。本研究结果表明，液泡可分为三类：（1）主要含有与新生自噬泡相关的松散膜盘绕的液泡；（2）含有紧密堆积膜并呈现阳性HRP反应的液泡，表明内吞和自噬途径的汇合；（3）由降解的膜结构组成并含有NADP酶活性反应产物的液泡，表明溶酶体与自噬体 - 内体发生了融合。高度发达的顺式、中间和反式高尔基体区室反映了胆囊上皮的生物合成和内吞活性。
