Pérez-Vendrell A M, Hernández J M, Llauradó L, Schierle J, Brufau J
Institut de Recerca i Tecnologia Agroalimentàries, Department of Animal Nutrition, Centre de Mas Bové, Reus, Spain.
Poult Sci. 2001 Mar;80(3):320-6. doi: 10.1093/ps/80.3.320.
One experiment was conducted using 960 1-d-old, sexed broilers of Ross 308 strain from 1 to 43 d to evaluate if one type of chemically isomerized marigold with 25% of xanthophylls as zeaxanthin (SME-25) could produce pigmentation equivalent to the current addition of conventional marigold with 10% of xanthophylls as zeaxanthin (SME-10) plus canthaxanthin (CTX) in practical broiler diets (maize-wheat-soybean). Birds were allocated in 32 pens, in a randomized complete block design (four blocks x four treatments). The treatments consisted of a nonpigmented control (T1), a combination of 35 ppm of yellow xanthophylls (YX) from SME-10 + 5 ppm of CTX (T2), a combination of 32 ppm of YX from SME-10 + 2 ppm of CTX (T4), and one treatment with 40 ppm of YX from a new SME-25 (T3). There were no significant treatment effects on chicken performance. All color parameters (Minolta coordinates, Roche color fan scores, Rank test) presented significant differences (P < 0.0001) because of dietary pigments on shanks and breast skin. Birds fed the SME-25 diet had less pigmentation than those fed equivalent quantities of a combination of SME-10 + CTX. The Minolta coordinate "b" measured in breast skin was a good indicator of YX content in feed, whereas the "a" coordinate measured on the shank showed a linear relationship with the dietary CTX level (r = 0.61, P < 0.0001). The same visual color classification of chickens was achieved irrespective of the rank test performed (by shank or carcass color). Lutein and zeaxanthin from the SME-25 product had lower deposition rates in skin and fat tissues than those from the SME-10 product. This finding seems to be related to the ratio of zeaxanthin stereoisomer RR (optically active) vs. RS that was found in tissues from the SME-10 product (97.8%:2.2%), whereas with SME-25 this ratio was 16.0:84.0%. These results suggest that inclusion of only the SME-25 product could not replace the current addition of SME-10 and CTX combinations.
进行了一项实验，使用960只1日龄、已鉴别性别的罗斯308品系肉鸡，从1日龄饲养至43日龄，以评估一种叶黄素含量为25%且玉米黄质为主要成分的化学异构化万寿菊（SME - 25）在实际肉鸡日粮（玉米 - 小麦 - 大豆型）中产生的色素沉着是否等同于当前添加的叶黄素含量为10%且玉米黄质为主要成分的传统万寿菊（SME - 10）加角黄素（CTX）。将鸡分配到32个鸡舍中，采用随机完全区组设计（四个区组×四种处理）。处理包括无色素对照（T1）、35 ppm来自SME - 10的黄色叶黄素（YX）与5 ppm CTX的组合（T2）、32 ppm来自SME - 10的YX与2 ppm CTX的组合（T4）以及一种含有40 ppm来自新型SME - 25的YX的处理（T3）。各处理对鸡的生产性能无显著影响。由于日粮色素的作用，所有颜色参数（美能达坐标、罗氏比色扇评分、秩和检验）在鸡腿和胸肌皮肤上均呈现出显著差异（P < 0.0001）。饲喂SME - 25日粮的鸡的色素沉着比饲喂等量SME - 10 + CTX组合的鸡少。在胸肌皮肤上测得的美能达坐标“b”是饲料中YX含量的良好指标，而在鸡腿上测得的“a”坐标与日粮CTX水平呈线性关系（r = 0.61，P < 0.0001）。无论进行何种秩和检验（通过鸡腿或胴体颜色），对鸡的视觉颜色分类都是相同的。SME - 25产品中的叶黄素和玉米黄质在皮肤和脂肪组织中的沉积率低于SME - 10产品中的。这一发现似乎与在SME - 10产品组织中发现的玉米黄质立体异构体RR（光学活性）与RS的比例有关（97.8%:2.2%），而SME - 25的这一比例为16.0:84.0%。这些结果表明，仅添加SME - 25产品不能替代当前添加的SME - 10和CTX组合。
