Namangala B, De Baetselier P, Noël W, Brys L, Beschin A
Department of Immunology, Parasitology and Ultrastructure, Flemish Interuniversity Institute for Biotechnology, Free University Brussels (VUB), St-Genesius-Rode, Belgium.
J Leukoc Biol. 2001 Mar;69(3):387-96.
The type I/type II cytokine balance may influence the development of different subsets of suppressive macrophages, i.e., classically activated macrophages (caMphi, type I) versus alternatively activated macrophages (aaMphi, type II). Recently, we showed that although mice infected with phospholipase C-deficient (PLC-/-) Trypanosoma brucei brucei exhibit a clear shift from type I to the type II cytokine production, wild type (WT)-infected mice remain locked in a type I cytokine response. In the present study, phenotype and accessory cell function of macrophages elicited during WT and PLC-/- T. b. brucei infection were compared. Results indicate that caMphi develop in a type I cytokine environment in the early phase of WT and PLC-/- trypanosome infection, correlating with inhibition of T cell activation triggered by a mitogen, a superantigen, or an antigen. In the late stage of infection, only PLC(-/-)-infected mice resisting the infection develop type II cytokine-associated aaMphi correlating with impaired antigen- but not mitogen- or superantigen-induced T cell activation.
I型/II型细胞因子平衡可能会影响抑制性巨噬细胞不同亚群的发育，即经典活化巨噬细胞（caMphi，I型）与替代性活化巨噬细胞（aaMphi，II型）。最近，我们发现，尽管感染磷脂酶C缺陷型（PLC-/-）布氏布氏锥虫的小鼠表现出从I型细胞因子产生到II型细胞因子产生的明显转变，但野生型（WT）感染小鼠仍保持I型细胞因子反应。在本研究中，比较了WT和PLC-/-布氏布氏锥虫感染期间引发的巨噬细胞的表型和辅助细胞功能。结果表明，在WT和PLC-/-锥虫感染的早期阶段，caMphi在I型细胞因子环境中发育，这与有丝分裂原、超抗原或抗原触发的T细胞活化受到抑制相关。在感染后期，只有抵抗感染的PLC（-/-）感染小鼠会产生与II型细胞因子相关的aaMphi，这与抗原诱导的T细胞活化受损相关，但与有丝分裂原或超抗原诱导的T细胞活化无关。
