Flo T H, Halaas O, Torp S, Ryan L, Lien E, Dybdahl B, Sundan A, Espevik T
Institute of Cancer Research and Molecular Biology, The Norwegian University of Science and Technology, Trondheim.
J Leukoc Biol. 2001 Mar;69(3):474-81.
Human Toll-like receptor 2 (TLR2) is a receptor for a variety of microbial products and mediates activation signals in cells of the innate immune system. We have investigated expression and regulation of the TLR2 protein in human blood cells and tissues by using two anti-TLR2 mAbs. Only myelomonocytic cell lines expressed surface TLR2. In tonsils, lymph nodes, and appendices, activated B-cells in germinal centers expressed TLR2. In human blood, CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The level of TLR2 on monocytes was after 20 h up-regulated by LPS, GM-CSF, IL-1, and IL-10 and down-regulated by IL-4, IFN-gamma, and TNF. On purified granulocytes, LPS, GM-CSF, and TNF down-regulated, and IL-10 modestly increased TLR2 expression after 2 h. These data suggest that TLR2 protein expression in innate immune cells is differentially regulated by inflammatory mediators.
人类 Toll 样受体 2（TLR2）是多种微生物产物的受体，可介导先天免疫系统细胞中的激活信号。我们使用两种抗 TLR2 单克隆抗体研究了 TLR2 蛋白在人类血细胞和组织中的表达及调控。只有骨髓单核细胞系表达表面 TLR2。在扁桃体、淋巴结和阑尾中，生发中心的活化 B 细胞表达 TLR2。在人类血液中，CD14+单核细胞表达的 TLR2 水平最高，其次是 CD15+粒细胞，而 CD19+B 细胞、CD3+T 细胞和 CD56+NK 细胞不表达 TLR2。单核细胞上的 TLR2 水平在 LPS、GM-CSF、IL-1 和 IL-10 作用 20 小时后上调，而在 IL-4、IFN-γ 和 TNF 作用下下调。在纯化的粒细胞上，LPS、GM-CSF 和 TNF 作用 2 小时后下调 TLR2 表达，而 IL-10 则适度增加其表达。这些数据表明，先天免疫细胞中 TLR2 蛋白的表达受到炎症介质的差异调控。
