Benham C J
Department of Biomathematical Sciences, Box 1023, Mount Sinai School of Medicine, 1 Gustave Levy Place, NY, NY 10029, USA.
Pac Symp Biocomput. 2001:103-14.
Stress-induced destabilization of the DNA double helix (SIDD) is involved in several mechanisms by which transcription is regulated. This paper describes a computational method for predicting the locations and extents of destabilization as functions of DNA sequence and imposed superhelical stress. This method is used to investigate several transcriptional regulatory events. These include IHF-mediated activation of gene expression in E. coli, the bimodal control of the initiation of transcription from the human c-myc gene, and the determination of the minimal requirements for transcriptional activity in yeast. Collaborations with experimental groups have established the central role of SIDD in each of these processes.
应激诱导的DNA双螺旋不稳定（SIDD）参与了多种转录调控机制。本文描述了一种计算方法，用于预测不稳定的位置和程度，作为DNA序列和施加的超螺旋应力的函数。该方法用于研究几种转录调控事件。这些事件包括大肠杆菌中IHF介导的基因表达激活、人类c-myc基因转录起始的双峰控制以及酵母中转录活性的最小要求的确定。与实验小组的合作已经确定了SIDD在这些过程中的核心作用。
