低剂量抗癌药物联合淋巴因子激活的杀伤细胞对卵巢腺癌细胞系SKOV3的细胞毒性作用

[Cytotoxicities of low dose anticancer agents combining lymphokine activated killer cell against ovarian adenocarcinoma cell line SKOV3].

作者信息

Chen R, Pan L, Zhou S

机构信息

Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 1999 Mar;34(3):172-4.

PMID:11263191

Abstract

OBJECTIVE

To investigate whether low-dose anticancer agents could increase the sensitivity of ovarian adenocarcinoma cell line SKOV3 to lymphokine activated killer cell (LAK).

METHODS

After SKOV3 cells were pretreated by low dose anticancer agents Taxol, cis-diamminedichloroplatin(CDDP), 5-fluorouracilum(5-FU) for 18 hours, the sensitivity of SKOV3 to LAK was detected by four 51Cr release assay. And the percentage of SKOV3 adhesion to LAK and intercellular adhesion molecule-1 (ICAM-1) expression on SKOV3 were detected by improved Grimm's assay and FACS respectively.

RESULTS

After pretreatment of SKOV3 cell with 1.5 micrograms/ml Taxol, 4 micrograms/ml CDDP, 25 micrograms/ml 5-FU or without anticancer agents as control for 18 hours, the cytotoxicities of Interleukin-2 activated LAK against them were 29.7%, 45.9%, 37.2% and 28.5% respectively. The conjugation rates of SKOV3 and LAK were 20.1%, 26.1%, 24.9% and 18.7% respectively. The positive rates of ICAM-1 expression were 52.5%, 65.5%, 68.1% and 49.7% respectively. CDDP and 5-FU increased ICAM-1 expression significantly and the sensitivity of SKOV3 cell to LAK cell lysis was well related to the ICAM-1 expression.

CONCLUSION

The results indicate that some low dose anticancer agents can increase the sensitivity of cancer cells to LAK cells and it would be useful in clinical practice.

摘要

目的

探讨低剂量抗癌药能否增加卵巢腺癌细胞系SKOV3对淋巴因子激活的杀伤细胞（LAK）的敏感性。

方法

用低剂量抗癌药紫杉醇、顺铂、5-氟尿嘧啶预处理SKOV3细胞18小时后，采用四甲基偶氮唑盐比色法检测SKOV3对LAK的敏感性。采用改良的格林氏试验和流式细胞术分别检测SKOV3与LAK的黏附率及SKOV3细胞表面细胞间黏附分子-1（ICAM-1）的表达。

结果

用1.5微克/毫升紫杉醇、4微克/毫升顺铂、25微克/毫升5-氟尿嘧啶预处理SKOV3细胞18小时或不做处理作为对照，白细胞介素-2激活的LAK对它们的细胞毒性分别为29.7%、45.9%、37.2%和28.5%。SKOV3与LAK的结合率分别为20.1%、26.1%、24.9%和18.7%。ICAM-1表达阳性率分别为52.5%、65.5%、68.1%和49.7%。顺铂和5-氟尿嘧啶显著增加ICAM-1表达，且SKOV3细胞对LAK细胞溶解的敏感性与ICAM-1表达密切相关。